Are you using the right disinfection methods in the production of edible mushrooms?
In nature, there are a large number of microorganisms in the air, water, soil, inside and outside plants and animals, and on various items. Preparation of edible mushroom strains, to use effective methods to inhibit the growth of other microorganisms, to create environmental conditions conducive to the growth of edible mushrooms, which requires us to disinfect all aspects of the production of edible mushrooms. Disinfection can be divided into three methods: low temperature disinfection, boiling water disinfection and chemical disinfection.
1. Low-temperature disinfection
Low-temperature disinfection is disinfection at a temperature of 60-75 degrees. Low-temperature disinfection can kill most of the microbial nutrient cells, such as the fermentation process of culture material is both a low-temperature disinfection process, which can effectively kill harmful bacteria and pests in the raw material.
2. Boiling water disinfection
Boiling water disinfection is boiling in boiling water for 30-40 minutes to kill the nutrient cells of microorganisms. To kill bacterial spores or mold spores, the boiling time should be extended for more than 2 hours, mainly used for disinfection of metal inoculation apparatus, syringes, etc.
3. Chemical disinfection
Chemical disinfection is the use of chemical drugs to kill microorganisms.
(1) Disinfectants commonly used for disinfection are: for fumigation and air disinfection mainly 37%-40% formaldehyde stock solution; for spraying ground and wall disinfection are: 5% solution of carbolic acid, 2%-5% solution of bleach, 1%-2% Lysol, Bordeaux solution (1:1 diluted configuration of copper sulfate and lime into a 100-200 times solution), etc.
Note: Try to exhaust formaldehyde gas when the operator enters the mushroom room.
(2) Disinfectants for inoculation room, instruments, mushroom wood surface, etc.: 70%-75% alcohol is commonly used for disinfection of table surface, instruments, mushroom wood surface, inoculation personnel's hands, etc. during inoculation, and anhydrous ethanol and 95% ethanol should not be used. In addition, 5% formaldehyde, 0.25% Neosporin, 0.1% mercury solution, 0.1%-0.2% potassium permanganate solution, etc. can also be used for disinfection.