Edible Fungus Liquid Spawn Inoculation Technology
Liquid strains have the advantages of convenience and quickness, short and consistent age, high purity, and strong vitality. They are more and more valued by researchers and producers of edible fungi. But how to use liquid strains correctly? There are a variety of inoculation methods in large-scale application liquids, each with its own characteristics, which are introduced below for reference.
1. Substrate surface inoculation method
This is a relatively common method at present. Most of the culture materials are bottled or bagged when inoculated, and there is a certain space at the upper end.
Such as the production of Flammulina velutipes, tea tree mushrooms, black fungus, Pleurotus eryngii mushroom packs, and the production of shiitake mushrooms, Coprinus comatus, Pleurotus ostreatus and other strains,When inoculating, spray the surface of the material with bacteria as much as possible. The liquid bacteria germinate and grow quickly, and the chance of contamination by bacteria on the cover is very small. Except for broken bags, the chance of contamination of the bacterial bag is extremely low, and even zero pollution can be achieved. The spheroids of this method grow well under the condition of sufficient oxygen.
2. Inoculation gun insertion inoculation method
The tip of the inoculation gun is made into a tube and inserted into the material for inoculation, and the bacteria balls grow in the material at the same time, which can shorten the germination time by more than half. In this method, one must pay attention to the supply of oxygen in the material (not suitable for pocketing), and the other must pay attention to the aseptic requirements of the inoculation environment, so as to avoid the bacteria not germinating or the tip of the pipette bringing in bacteria to cause pollution. For example, even if the mushroom sticks are not sealed when they are inserted from the center of both ends in the production, the strains in the middle of the sticks are not easy to germinate. This method inoculates a large number of mushrooms such as shiitake mushrooms, Coprinus comatus, Flammulina velutipes, and Oyster mushrooms in low-temperature seasons. The yield is 98% to 100%, and the time is shortened by more than half.
3. Pricking hole inoculation method on the bag surface
Inoculation of long bacteria sticks from both ends can not shorten the time of bacteria cultivation. Use a gun tip to pierce 3~5cm deep from one or both sides of the bag surface and inoculate at the same time. The hole interval should be determined according to the needs. For example, each stick of shiitake mushrooms should pierce 3~5 holes . This method adjusts the inoculum size and the distance between the holes to shorten the time of bacteria cultivation and reduce the contamination of bacteria. The disadvantage is that the amount of seed used is large, the inoculum amount of each well should not be less than 10mL, and the amount of inoculation that is not sealed after inoculation should reach more than 20mL per well.
4. Intra-bag spray inoculation
The long bacteria stick can also be inserted into the bag film from both ends and one side with the tip of the gun, and the bacteria are sprayed from between the material and the plastic film (the liquid pressure is required to reach 9.8×100KPa or more).
After spraying, press and disperse the bacteria by hand, so that one side of the bacteria stick is covered with bacteria, and after the mycelium grows 1~3cm, holes can be pricked to increase oxygen. This method can cover a larger surface of the material, and the microporous pollution on the surface of the bacteria fast bag is small. The disadvantage is that the amount of seeds used is large, and the amount of seeds used for each spray is about 100mL. And pay attention to reducing the water content of the compost when batching. Practice has proved that this method is more successful in the production of shiitake mushrooms.
The resistance of liquid strains to adverse environments is not as good as that of solid strains (for example, the ability to withstand hypoxia is not strong), and it is often difficult to succeed in the cultivation of fermented materials, raw meal cultivation or open mixing of plastic bag clinker. The advantages of liquid strains can only be brought into play by the good combination of liquid strain production technology and edible fungus cultivation technology.