Essentials of Phellinus sibiricum Cultivation Techniques
1. Cultivation time arrangement
The SH2 strain can be used to make the first-level seed around November 20 of that year, and the strain will be full after 10 days. At the beginning of December, the bacteria will be directly transferred to the secondary bacteria culture material, and the bacteria bottle will grow in about 30 days. At the beginning of January of the following year, the production of tertiary bacteria was carried out, and the strains grew for 45 days to fill the bag. During the growth of the tertiary bacteria, prepare the poplar section wood, split sections, splicing, bagging, and tying at both ends (the size and thickness can be determined according to the actual situation) to facilitate the inoculation of both ends. Section wood inoculation will be carried out around mid-February, buds will be urged and put into the shed at the beginning of April, and then fruiting management will be carried out. Phellinus igniarius is perennial and can be harvested in 3 to 5 years.
2. Preparation of culture medium (material)
The formula of the first-level culture medium: 20 grams of five-dimensional glucose, 5 grams of peptone, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 24 grams of agar, and 1000 ml of water.
Secondary seed material formula: 77% sawdust, 15% bran, 5% corn flour, 1% five-dimensional glucose, 1% potassium dihydrogen phosphate, 1% gypsum. The water content of the culture material is 65%.
Three-level seed material formula: 80% sawdust, 18% bran, 1% five-dimensional glucose, 1% gypsum. The water content of the culture material is 65%. Choose poplar as the substrate for the cultivation section of wood.
3. The amount of inoculation
A first-level test tube can be transferred to 6 bottles of second-level strains; a bottle of second-level strains can be transferred to 25 bags of third-level strains; a bag of third-level strains can be connected to sections of wood 1 part. When calculating the materials for cultivation, the preparation of raw materials can be carried out by the reverse method.
Using high pressure sterilization, the sterilization conditions of the first-level bacterial material are 121°C for 20 minutes; the sterilization conditions of the second-level bacterial material and the third-level bacterial material are both 121°C and 120 minutes. Sterilization of section wood can be controlled at 121°C for about 4 hours. If conditions do not permit, you can purchase first-level, second-level or third-level bacteria from a qualified unit, and the section wood can be sterilized for 12 hours under normal pressure sterilization conditions.
5. Cultivation temperature and ventilation management
The cultivation of secondary strains, tertiary strains and section woods needs to be carried out within a certain temperature range, and reasonable ventilation is required to ensure that the hyphae of Phellinus igniarius grow well and maintain activity, which is conducive to fruiting. Cultivation indoors, the growth of mycelium does not require light, it should be cultivated in the dark, and the temperature is controlled at 25-28°C. Ventilation is not required in the early stage of the culture. In the middle and late stages of the culture, as the mycelium fills the bottle (bag), ventilate 2 to 3 times a day, and the effective ventilation time is controlled at 1 hour each time.
Sixth, section wood into the shed preparation
During the cultivation of Phellinus igniarius mycelium, build the cultivation arch shed, dug the reservoir, and enter the shed with water spraying equipment. The arch canopy is covered with 2 to 3 layers of sunshade nets. Reserve vents, but temporarily close them, and then use them when you need to increase the amount of ventilation in the later stage of cultivation. The shed is used as a border, and the border is slightly higher than the water spray aisle. Determine the number and width of the borders according to the size of the shed and the amount of cultivation. Leave 20 cm for the placement gap. The border surface is padded with sand and stone to facilitate water filtration. When making borders, the middle is slightly higher than the two sides. After the cultivation shed is ready, it is disinfected every two days and ventilated. After 3 times of disinfection, the bacteria section can be put into the shed to adapt to the environment. Two days after entering the shed, a circular incision is made on the bacterial segment, 15-20 cm long and 1-1.5 cm wide. When placed, the bottom of the bacteria section is taken off the bag and stands in the border. Ensure that the lower end of the incision does not separate from the bacterial skin as much as possible, so as to ensure that no water accumulates at the lower end of the bag incision during the water spray process, which plays a role in protecting the bacterial segment.
7. Fruit body cultivation management
Temperature management: After the bacterial segment enters the shed incision, the air can be ventilated twice a day in the early stage for 1 to 1.5 hours each time, and the amount of ventilation can be increased in the later stage, and the vents can be opened to ensure that the air is ventilated 3 to 4 times a day, and the ventilation is 1.5~ 2 hours. During the day, the temperature should be controlled at 28～34℃, and the highest should not exceed 35℃, otherwise Phellinus linteus will lignify in advance and stop growing. When the temperature is too high during the day, it is necessary to strengthen ventilation and humidify and cool down. It is natural temperature at night, 18～22℃.
Humidity management: Before germination of Phellinus linteus fruiting bodies, the ground must be moist, with a relative humidity of 90% to 95%. When spraying water at the incision, it is required to spray less water, lightly spray water, spray water repeatedly, spray water 5-7 times a day. After the fruit body germinates, the amount of water spray should be appropriately increased, and the number of water sprays should be reduced. Spray more water on sunny days and less water on cloudy and rainy days. In order to keep the incision moist in the early stage, the arch canopy needs to be closed and properly ventilated. It is necessary to increase the amount of ventilation in the later period, and then seal the arch shed after ventilation. After the growth of Phellinus igniarius fruiting bodies, the surface is not smooth, showing bright yellow and horseshoe-shaped. If the surface color of the fruit body changes and there is no bright yellow, it indicates that the fruit body has stopped growing. At this time, preparations should be made before overwintering.