A. Sawdust 78%, bran 20%, sucrose 1%, gypsum powder 1%.
B. Cottonseed hull 80%, bran 18%, sucrose 1%, gypsum powder 1%.
2. The preparation method of cultispecies and sack: mix materials in accordance with the proportion of 1 to 1.1 or 1.2 (Material to water), then bagging (bottling). Sterilize for 2 hours under the condition of high pressure and high temperature. Under 100 degrees, you should sterilize for 12-16 hours or even longer. After cooling, inoculate in inoculation room or inoculation box, before inoculation, it is needful to fumigate and sterilize in inoculation room or box with Chlorine dioxide disinfector for 4-6 hours, the dosage is 0.25 to 1.0mg per cubic meter. In usual, each stock strain inoculates 30 bags of cultivation strains, each bag of cultivation strain inoculates 20-30 bags. After that, put them into cultivation room with the temperature of 22-25 degrees.
Before the cultivation, the room should be fumigated and sterilized by Chlorine dioxide disinfector, the dosage is 0.25 to 1.0mg per cubic meter. The temperature of the cultivation bag should be controlled in a range of 22-25 degrees. Moreover, spawns are easy to age in too high temperature.Mushroom bags become mature in about 30-40 days.
When you find that the color of mushroom bag has changed from white to yellow brown or even become tuberculate, then the temperature should be kept in about 20 degrees, water moisture should be moderately increased while the relative air humidity should be kept in a range of 80%-85%, ventilation should be strengthened and certain scattered light should be given to promote color-changing, next, induce the formation of mushroom buds and create the initial condition for fruiting. 3. The management during the fruiting period: during the fruiting period of Shiitake, the temperature should be kept in a range of 10-15 degrees, relative air humidity should be kept in 85%-90%, sufficient scattered light should be given while moderate ventilation condition should be created.